FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23115656
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG87000.047.59425794
CAAGTCG64400.023.86490694
TAAGTCG30300.023.11221194
GAAGTCG110250.016.92426594
GTCCGAT14700.012.7891171
GTTCGAT28300.012.7879871
GTCGTTT19550.012.501281
CGTAACG5353.9926454E-711.4205623
AAAGTCG41600.011.29807994
TCGGATT27200.011.0588242
CGCGATT8652.0008883E-1110.8670521
GTTTATT116750.010.7888661
GGCGATT41600.010.62019351
CTCGGTT37600.010.3751
CGTATCG18850.010.2228123
GTCGGTT51850.010.1523631
CTCGGAT63750.010.0266671
CTCGTAT16450.010.0000011
CGGGAAT56400.010.01
CGCGGAT24000.09.98751
Produced by FastQC (version 0.11.5)