FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22455577
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG127700.055.31793294
CAAGTCG91850.031.31627794
TAAGTCG37650.022.96945494
GAAGTCG140100.021.73875894
TATACGC8800.017.0909087
AAAGTCG54150.017.01200394
ATATACG12350.015.9838066
CGCGTAT5103.6379788E-1215.6666651
TACGCTA10650.015.0046949
CGAAAAT36800.013.7934781
GTTTAAT87150.013.75215151
GTCGGTT39850.013.2095341
CGCAAAT31900.012.9655171
TTTTTTC312700.012.625522
GTTTATT95650.012.3826451
CTTTATT164650.012.2745211
CTTTAAT103550.012.2095591
CGGAAAT49700.012.1991951
GTTTTTT257700.012.1284431
GTTTTAT141850.011.8618261
Produced by FastQC (version 0.11.5)