FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22455577
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG164450.043.64183894
CAAGTCG111350.026.08531694
TAAGTCG40850.023.93145494
GAAGTCG142050.019.4551294
TATACGC11050.018.7149317
TACGCTA12100.018.6446299
AAAGTCG62600.016.51757294
ATATACG13000.015.9076916
CGAATCG14700.014.3877553
CTTTAAT103100.012.71871951
AATCGTA18350.012.2942775
CGCAAAC47500.012.1705261
GTTTTTT269750.011.9699721
CGAAGAT84750.011.7569311
CTTTATT162750.011.6958531
GTTTTAT129800.011.5870561
ATACGCT19600.011.5102028
CGAAAAT44600.011.4865471
GGAAAAC143150.011.4257771
GTTTAAT79550.011.3438081
Produced by FastQC (version 0.11.5)