FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21950318
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG88650.050.89678694
CAAGTCG62850.026.69689694
TAAGTCG27900.021.22580594
GAAGTCG106600.018.65009394
TACGCTA6750.017.4074089
TATACGC6950.016.9064757
GTTTTTT318850.016.7009561
ATATACG9850.016.2233496
CGAAAAT41750.015.6479031
GTTTAAT101200.014.6294461
TTTTTTC374550.014.355362
GCGAAAT30150.014.1857381
AAATCCA208400.013.6444334
AAAGTCG42700.013.2084394
AAAAATC244550.013.0881212
CTTTAAT109550.012.9995441
TTTTTTG432200.012.9407682
GTTTTCT217300.012.9341931
CGTATCG17850.012.63865650-51
GAAAAAT248750.012.5270361
Produced by FastQC (version 0.11.5)