FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21950318
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG116450.038.6251694
CAAGTCG89650.021.86168394
GAAGTCG104950.018.40590994
TAAGTCG30650.016.56117494
GTTTTTT339900.015.5698751
CGCATAT8650.015.2138731
TATACGC7300.014.8082197
TACGCTA7400.014.6081089
ATATACG10650.014.5633796
GCGAAAT28850.014.3362221
CGAAAAT52050.013.9058591
GTTTAAT91700.013.3773161
CGCAAAT32050.013.1981281
CGCGTTT15350.013.1661231
CGAAAAC32900.013.1428581
CTTAGAT61250.013.1216321
AAAAATC220450.013.1118162
GTTTTAT166850.012.9014081
CTCGATT48800.012.6168021
CGTATCG18150.012.55922850-51
Produced by FastQC (version 0.11.5)