FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22958491
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG102850.053.78610294
CAAGTCG93700.030.3970194
TAAGTCG27200.022.80882594
GAAGTCG118450.019.20472794
AAAGTCG44150.014.05209594
GTCGGAA39100.012.2608691
GTCCGAT14850.012.0269371
CGCGTAA7057.2759576E-1112.0000011
GTCGGAT29550.011.2927251
GGCGGTT57350.011.22755051
AATCGTA21350.011.2271675
ATCGTAT16900.011.1242616
GTCGGGT44000.010.6818181
GTCGAAA30650.010.580751
GTTCGAT26800.09.9962691
GTAGGTC85150.09.9906048
GTCGGAG38650.09.97153951
CGCTTAA31250.09.926444-45
GGGAAAT159750.09.88544751
TACTCGC55600.09.84802262-63
Produced by FastQC (version 0.11.5)