FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22958491
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG126650.044.19818594
CAAGTCG110700.027.63956894
TAAGTCG26500.027.3132194
GAAGTCG120100.016.90591294
CGCGTAA5754.474714E-1013.078261
GCGGTAT20900.012.1435411
AAAGTCG48850.011.64176294
GGCGATT38000.011.6263161
GTCCGAT13550.011.4464961
AATCGTA20600.011.4077675
GGCGGAT87950.010.8482081
CGCTTAA31500.010.81746144-45
GGCGGTT55200.010.8134061
GGGAAAT153450.010.7507341
GCCGGTT52200.010.714561
ATCGTAT18100.010.1270726
GTTCGAT25850.010.0000011
CGCGGAT25600.09.91406251
CGGGAAT52350.09.8758361
GTCGGAT30550.09.8461541
Produced by FastQC (version 0.11.5)