FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23018555
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG86050.042.9308594
TAAGTCG27950.021.69230794
CAAGTCG72250.020.62145294
CGAAAAT39900.016.4912261
GTTTTTT308550.015.3087011
TTTTTTC426700.014.8919612
GAAGTCG105300.014.81861494
TTTTTTG480250.013.5543992
GCGAAAT29100.013.4054981
GTTTTAC71050.012.9655171
CTCGATT44400.012.5968461
TATACGC8600.012.5697677
TACGCTA8250.012.5333339
TTTTTTA433950.012.3253832
GTCGAAT14150.012.2897521
AAATCCA204250.012.1268054
GAAAAAT228600.012.0686791
GGAAAAT178300.011.914751
GTTTTCT238550.011.900231
TTAGCGA17850.011.848747
Produced by FastQC (version 0.11.5)