FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23018555
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG112900.032.7626294
TAAGTCG29500.018.64067894
CAAGTCG81900.018.30646994
GTTTTTT307000.014.8501621
CGCAAAC44750.014.7039111
GAAGTCG110800.014.54963994
AAATCGA34050.014.3553594
CGCGAAC17250.013.6231881
CTCGATT45700.013.2669571
GTTTAAT92950.013.0962871
CTTAGAT62100.013.0933971
CGAATCG17500.012.8914283
CGAAAAC36600.012.841531
TTAGCGA20050.012.6583537
CGGATAT23250.012.1290311
CGCAATT22500.012.1155551
AATCGTA19350.011.9018085
GGAAAAT179650.011.8252141
GTTTTAC68550.011.7242881
AAATCCA198150.011.5039114
Produced by FastQC (version 0.11.5)