FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21964174
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTTTC513700.018.3352132
ACTCGAA47450.016.24446794
TTTTTTA551650.015.7106852
GTTTTTT368150.015.473041
TTTTTTG581850.015.3233652
CGAAAAT50050.014.7432571
TTAGCGA34850.014.4304157
TAGCGAG35400.013.9406788
AAGTCGG45100.013.75609794
TATACGC6157.2759576E-1213.7560967
TATCGGA28600.013.4755235
CGCAAAT29300.013.4744011
TTATCGG29750.013.1126044
CGAAGAT88850.013.0129421
GGAAAAT188150.012.9396751
CGAGAAC60900.012.8883411
AAATCGA31050.012.86634354
GGAAAAC163800.012.7973131
TTTTTGA301450.012.7848734
ATTTACG61950.012.7457626
Produced by FastQC (version 0.11.5)