FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21964174
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGC7650.017.2026147
CGCAAAT27100.015.7822871
CGCAAAC40100.014.6508711
GTTTTTT373100.014.6378981
CGAAGAT83400.014.4832121
CGAAAAT56750.014.1621141
CATATCG21700.013.8617513
ACTCGAA43150.013.8331494
GCGAAAT30700.013.7785011
TACGCTA9250.013.210819
TTAGCGA29000.012.8034487
ATATACG11400.012.7807016
CGAGAAC57950.012.733391
CTTAGAT63650.012.5530241
GTTTAAT113150.012.544411
CCGGTTA28000.012.337544-45
CTCAGAT160350.012.2812591
TAGCGAG29900.012.2608698
CGCATTC45100.012.1929041
CGAAAAC29300.012.1911251
Produced by FastQC (version 0.11.5)