FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22743379
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG94750.043.25488394
CAAGTCG76400.026.14528794
TAAGTCG27100.022.89298894
GAAGTCG119100.016.37699394
TACGCTA7150.013.8041949
GTCGGTT46650.012.7952831
AATCGGG43000.011.9139545
AATCGTA19500.011.3282055
GGAAAAT171050.011.2657111
CGAAAAT39700.011.2468511
GTTTATT91800.011.1100221
CGCGTAT5505.708407E-711.109091
CGGAAAT42450.010.9611311
GTCAATT56450.010.9069971
GGGAAAT155550.010.8775311
CGAAGAT89050.010.8725421
ATATACG10850.010.8294936
AAAGTCG46600.010.79184594
TATACGC8802.910383E-1110.6818177
CGCAATT20250.010.6765431
Produced by FastQC (version 0.11.5)