FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22743379
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG122350.033.8430794
CAAGTCG91250.024.05369894
TAAGTCG28500.019.12982494
GAAGTCG116300.014.91229594
CGGAAAT44050.013.3371171
AATCGGG38450.013.0793245
AAAGTCG52300.012.40152994
GTCGGTT46050.011.7372411
GGAAAAT166150.011.5696651
CGCGAAC15100.011.5165561
TTAGCGA21450.011.1748257
CGAAAAT44150.011.0713471
CAATCGG31800.010.9371064
CTCGGAT67000.010.8731341
CGCGTTT14100.010.6666661
GGCGAAT42400.010.6415081
GCGGAAT41150.010.6221131
GTTTATT98600.010.4868151
GCGAAAT27900.010.4444441
AATCGTA18450.010.4444445
Produced by FastQC (version 0.11.5)