FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21692582
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG95250.054.32755794
CAAGTCG76150.027.09520394
TAAGTCG27600.024.86231894
GAAGTCG106250.018.13646994
GTTTTTT301500.015.9628521
GCGAAAT26650.015.5196981
GCCGAAT47950.014.5067781
AAAGTCG43600.014.22935894
TTTTTTC398700.014.1459742
TTTTTTG422900.013.9810822
GTCGAAT14150.012.6219071
GTTTTAC66800.012.594311
GAAAAAT224100.012.5836671
AAATCCA194900.012.5397634
CGCGAAT14550.012.2749141
AAAAATC229550.012.1415812
CTCGATT31550.012.0665611
GGAAAAT172050.011.9651261
GTTTAAT93900.011.9627271
GCGGAAT44300.011.7765241
Produced by FastQC (version 0.11.5)