FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21692582
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG116600.044.13807794
CAAGTCG89500.023.89385294
TAAGTCG27150.023.54327694
GAAGTCG111300.017.14465394
GCGAAAT27350.017.0127941
GTTTTTT303800.015.0684661
CGCGTAT4202.6635462E-713.4285711
CGAATCG14150.013.2862193
AAAGTCG51400.013.07587594
CGAAAAC32950.012.6949921
GCCGAAT45300.012.5540841
CTCGATT33550.012.1877781
CGCGAAT10950.012.0182641
CGCGTTT15700.011.9745231
AAATCCA189300.011.8182774
GCGGATT24750.011.7737381
CGAAAAT51850.011.6026991
CGCAAAC43250.011.5190741
GCCGATT23350.011.4732331
CGCTAAT9900.011.3939391
Produced by FastQC (version 0.11.5)