FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21684542
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG147950.051.7174794
CAAGTCG106800.025.48033794
GAAGTCG137450.023.55984194
TAAGTCG43950.022.35039994
AAAGTCG63650.019.78947494
CGCGAAT19300.018.0207231
AAATCGA35100.017.943024
TTCGCTC46050.017.554832
AATCCAA114950.014.8829935
AATCCAC111250.014.2373035
CGAATTG27650.014.1084993
TTCAATC98350.014.0498232
GCGAATT23500.013.82
CAAATCG30350.013.78253653
TTTAATC108400.013.6577492
AAATCCA194400.013.6358024
TTTGCTC144500.013.4332182
CGGCAAT43350.013.010381
CAATCCA88950.012.9454764
AATCGGG43300.012.916865
Produced by FastQC (version 0.11.5)