FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22494094
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG134200.048.12071694
CAAGTCG111450.027.7909494
TAAGTCG30900.024.48867494
GAAGTCG124800.019.77163594
AAAGTCG55750.012.645740594
CGCGTAA5851.0426629E-711.2478631
CGCTTAA31100.011.18328144-45
GTCCGAT16050.011.1277261
GTTCGAT27450.010.7868861
AATCGTA20750.010.6457835
TCGGATT25600.010.4648442
GGGAAAT155000.010.0367751
GTCGAAT13300.09.8947371
GCCGGTT46150.09.8786571
GGCGGTT53400.09.8576781
CGCGGAT25850.09.8181831
GGCGAAT34550.09.6584661
GAATCGG38100.09.4986894
CGGGAAT53850.09.4261851
CGGAAAT40450.09.411621
Produced by FastQC (version 0.11.5)