FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22255014
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG128000.037.9304794
CAAGTCG97000.024.37216694
TAAGTCG31700.019.27444894
GAAGTCG117050.018.06920294
CGAAGAT81950.012.9042091
AAAGTCG57200.012.40734394
TATACGC7853.6379788E-1211.9745237
CGGAAAT46450.011.7373521
TACGCTA9400.011.59
ATATACG10800.011.3148156
GGAAAAT174550.011.2552281
CGCAAAT31800.011.2327041
AATCGTA16950.011.0914455
CGAAAAT39500.010.9468351
CGAATCG14600.010.9452053
GTCGGTT38200.010.8272251
GTTTAAT79400.010.7732991
CGGACAT55250.010.6334841
GTTTTTT278650.010.5418981
GGAAAAC153150.010.4955931
Produced by FastQC (version 0.11.5)