FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22442776
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG164100.050.89518494
CAAGTCG130650.031.2973694
TAAGTCG35550.027.23487994
GAAGTCG143100.020.85604594
AAAGTCG58850.014.69498794
GTTCGAT33100.013.9154081
GTCCGAT16500.013.1030291
TTCGATT35450.012.7277842
TTAGCGA25000.012.4088
AATCGTA17650.012.2492915
TCGGATT27150.012.1178632
GTTTATT97150.011.4173961
GCGGTAT23000.011.0347821
GCCGGTT57750.010.9870131
TAGCGAG31050.010.7471819
CGCGGAT28750.010.6260861
TATACGG16000.010.57534-35
CTCGGAT64200.010.4688461
GGCGATT44300.010.0790061
CTCGGTT39000.09.8820511
Produced by FastQC (version 0.11.5)