FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23103249
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG89550.045.18927894
CAAGTCG73450.023.5479994
TAAGTCG28250.019.96460294
GAAGTCG115400.016.6984494
GTTTTTT297650.015.1113721
CGAAAAT38150.015.0301441
TTTTTTC427800.014.2494152
TACGCTA9500.013.8526319
CTTAGAT69250.013.5061381
TTTTTTG471400.013.1308862
CGCGATT7550.013.0728471
CGCATAT10950.012.8767121
CGAATCG16850.012.830863
CGGAAAT44700.012.8277411
TATACGC10350.012.7149757
GGAAAAT191500.012.6887731
TTTTTTA446450.011.8329042
CGAAAAC35450.011.7997181
GTCGATT20900.011.6937791
AAATCCA204100.011.67515854
Produced by FastQC (version 0.11.5)