FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23103249
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG112750.035.51574394
CAAGTCG77950.019.95766694
TAAGTCG31550.019.36608794
GAAGTCG111900.016.42269994
CTCGATT40000.014.68751
CGAAAAC36700.014.3433241
GTTTTTT311200.013.7133671
CGGAAAT47500.012.8631571
AAATCGA34300.012.6064154
GCGAAAT28250.012.3115041
GTTTTAT166500.012.0816821
GGAAAAT195300.012.0327711
AATCCAA125450.011.988845
CGAAAAT45550.011.9692651
AAATCCA210950.011.7639264
CTTAGAT64000.011.751
CGCAAAT32900.011.7142861
CTCAGAT192750.011.6311291
GAAAAAT233000.011.578541
GTTTAAT93650.011.5429791
Produced by FastQC (version 0.11.5)