FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21611303
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG109950.041.25057294
CAAGTCG89800.022.55790794
TAAGTCG27000.020.36666794
GAAGTCG106000.014.85377494
GTCCGAT13100.012.5572521
ATCGTAT13700.012.0072996
AATCGTA19600.011.9897965
AAAGTCG40700.011.54791294
GTTCGAT29300.011.2286691
GTCGTTT21100.011.1374411
TCGGATT23150.010.9632832
CGCGGAT23800.010.4663881
TAGCGAG26000.010.3038459
CGTATCG17800.010.2977523
CTCGGAT52850.010.1381271
CCGGTTA19200.010.0364582
TATACGG16650.09.73873934-35
TTAGCGA24300.09.6707828
GCCGGTT53650.09.6365331
GTCGGAT28000.09.5678571
Produced by FastQC (version 0.11.5)