FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22997572
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG103900.046.36669594
TAAGTCG30300.024.66336494
CAAGTCG85550.022.14026694
GAAGTCG126900.016.96296194
GTTTTTT282950.015.016081
AAAGTCG48150.014.64174494
TTTTTTC411250.014.5828562
GTTTTAC72400.014.5414361
CGAAAAT38800.014.5360811
TTTTTTG455750.013.7674162
GAAAAAT220900.013.0851071
CGCAAAT28750.012.9147821
TACGCTA8750.012.8914289
AAATCCA199850.012.6760064
GTTTAAT90000.012.5333331
CTTAGAT69850.012.38081551
GCGAAAT26450.012.0831761
TTTTTTA409650.012.0812882
CGCGAAT10550.012.0284351
GTTTTAT153650.011.96029951
Produced by FastQC (version 0.11.5)