FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22997572
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG134750.036.1001894
CAAGTCG96450.021.87972894
TAAGTCG33200.017.83734994
GAAGTCG130300.016.30391394
CTTAGAT69200.014.8742771
GTTTTTT278350.014.1666971
GTTTAAT88250.013.6339941
TACGCTA9700.013.567019
CGCAAAC48350.013.4146841
TATACGC8900.012.6741577
CTCGATT48700.012.54621
CTCAGAT165750.012.5049761
CGAAAAC39250.012.3337581
CGGAAAT45000.012.3244441
CGCGAAC18350.012.2942781
GGAAAAT181100.012.1976811
CGCAAAT29300.012.1911251
CGGATAT22050.012.1496591
AAATCGA34100.012.1290314
TCGGATT26100.012.0651342
Produced by FastQC (version 0.11.5)