FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21071142
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG146100.050.86036794
CAAGTCG107250.028.3533894
TAAGTCG35800.027.70111794
GAAGTCG136300.023.55172394
CGCGTAT4251.9777872E-814.3764711
AAAGTCG53050.014.17530794
GTCGGAT36350.013.83493894
GTCCGAT14750.013.3830511
CGCGTAA6101.4999387E-811.5573781
CGTATCG15750.011.0412693
CTCGGTT34600.011.0028911
CGAATCG12100.010.8760333
CGCGATT5801.1315897E-610.5344831
TCGGATC42050.010.5065494
GTTCGAT24750.010.4444451
GTTTATT103800.010.3689791
TCGGATT23400.010.0427352
GTCCGTT21050.09.600951
ACAAGTC120550.09.59104194
GTCGGTT40250.09.5751541
Produced by FastQC (version 0.11.5)