FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22284495
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG109150.050.8538794
CAAGTCG86850.026.46286894
TAAGTCG34200.024.18713694
GAAGTCG118950.018.33375494
CGAATCG13550.016.996313
TATACGC9350.015.582897
ATATACG12950.014.5173766
TACGCTA8500.014.3764719
GTCGGTT46250.014.3286491
AAAGTCG55250.013.27058994
CGCGAAT11650.012.5064381
GTTTTAC76800.012.4843771
AATCGTA19700.012.4060925
GTCGGGT61300.012.2675371
AATCGGG38650.012.1604155
CGCGGAT24150.012.0662541
CGAAAAT34450.011.8693761
CAATCGG27050.011.6414064
GTCGGAT34400.011.6133731
CGGGTCT123650.011.2891233
Produced by FastQC (version 0.11.5)