FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22007958
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG91600.049.2576494
CAAGTCG71350.023.77995794
TAAGTCG31350.019.63955194
GAAGTCG106100.018.16211194
TATACGC9900.018.0404037
TACGCTA9850.016.7005069
GTTTTTT346200.015.3136891
ATATACG14250.014.8421056
AAATCGA35450.013.9210144
AAAGTCG44900.013.39866494
TTTTTTC461500.013.1681462
CGAAAAC39100.013.1023021
GTTTAAT103650.013.0593341
GTTTTTC195500.012.9099741
GTCGATT21600.011.9675911
AAAAATC267400.011.9169772
GCGAAAT25450.011.8192521
GTTTTAC86050.011.79779151
AAATCCA213850.011.7582414
CTTAGAT77450.011.7120721
Produced by FastQC (version 0.11.5)