FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22007958
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG115850.038.98748494
CAAGTCG82200.022.52797994
TAAGTCG34000.018.38529294
GAAGTCG105750.016.35555594
CGAAAAC41950.015.1251481
GTTTTTT363300.014.83871
AAATCGA34800.014.58620554
GTTTAAT105550.013.6702991
CTCGATT45050.013.1453931
CTCAGAT177200.012.9170431
GCGAAAT24550.012.8268821
CGCATAT10300.012.7766981
AAATCCA221850.012.7536624
CGCAATT20450.012.6405861
TATACGC9700.012.5979387
CTTAGAT75900.012.4466391
CGGAAAT41450.012.3594691
GTTTTTC202650.012.1993581
GGAAAAT195700.012.1282581
AAAGTCG54000.012.09814794
Produced by FastQC (version 0.11.5)