FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21957846
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG136650.056.33809394
CAAGTCG102800.028.34630694
TAAGTCG34300.024.25364594
GAAGTCG138250.024.10343794
AAAGTCG50250.020.3900594
GCGAAAT29000.018.1517241
TTTTTTC359000.017.6348192
GTTTTTT254800.016.6197011
CTCGATT33300.014.5375381
TTTTTTG369750.014.3764712
CCCGAAC58600.013.9556311
GCCGAAT55550.013.8757881
CGCTAAT7850.013.1719751
TCGATTG21850.012.9061782
CGAAAAT32250.012.8248061
AAATCCA172800.012.8107644
GTTTTTC141100.012.7909291
GTTTTAC51850.012.6904541
TTTTTTA344250.012.6425562
AAAAATC179100.012.5700722
Produced by FastQC (version 0.11.5)