FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22175914
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG104600.053.2906394
CAAGTCG81800.026.25794494
TAAGTCG33150.021.97586894
GAAGTCG117050.018.39043294
AAAGTCG45950.015.03590894
GTCGGTT40900.014.9388741
TATACGC7900.013.0886077
CGCGATT6001.1745215E-811.7499991
GTTTATT111200.011.5386691
CGCAATT22450.011.5144771
GGCGAAT31650.011.285941
GTTTTAT159650.011.2164121
CGAATCG14750.011.1525423
CGCAAAT31850.011.0675041
GGAAAAT207950.010.8487621
TACGCTA6958.8348315E-910.8201449
GTTTTTT297250.010.7835151
GTCGAAC42350.010.7650531
CTCAGAT178400.010.5381161
CGCAAAC35250.010.5333341
Produced by FastQC (version 0.11.5)