FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22508995
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG121750.036.21026694
CAAGTCG88500.022.1988794
TAAGTCG32000.019.38749994
GAAGTCG110650.016.05603294
CGAAGAT82750.013.6314191
CGAAAAT39500.012.8506321
CGCGTAT4153.4067853E-612.457831
TACGCTA9250.011.6864869
AAAGTCG55250.011.48416294
GTTTATT108550.011.4306761
GGAAAAT173050.011.3799471
CTCAGAT175800.011.1484631
GTTTAAT82650.011.0889281
AATCGTA18100.010.9060775
CGCAAAC41200.010.83737851
CTTTAAT104100.010.7002881
GTTTTTT274000.010.6864951
GTCGGTT41350.010.5707381
CGCGGAT26850.010.5027931
CGGAAAT40550.010.4315651
Produced by FastQC (version 0.11.5)