FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22350057
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG116100.055.01550394
CAAGTCG84250.030.0130694
TAAGTCG34150.024.36017894
GAAGTCG135850.021.03496794
GTTTTTT289200.015.2116181
TTTTTTC367450.015.0292572
CGAAAAT36250.014.7806891
TACGCTA9150.013.8688539
AAAGTCG51750.013.80483294
TTTTTTG399500.013.2588242
CGAAAAC33900.012.6165191
CGCAAAT30750.012.5333341
AAATCGA33500.012.4865684
TATACGC8700.012.4252877
ATATACG11350.012.4229086
AAAAATC215450.011.95451452
GAAAAAT218950.011.9351451
GGAAAAT169450.011.8436131
CGCGAAT21900.011.8036541
GTTTTCT220200.011.7820181
Produced by FastQC (version 0.11.5)