FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22350057
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG135950.047.0172994
CAAGTCG101900.027.5358294
TAAGTCG32950.025.10470494
GAAGTCG125050.019.20591794
AAAGTCG56850.014.88126894
GTTTTTT274050.013.6172241
CGAAAAT43850.013.2907661
TACGCTA8700.012.4252879
TATACGC9500.012.3684227
CTCGATT37750.011.8278151
AAATCCA205450.011.7814564
GTTTAAT84550.011.7291551
GGAAAAT169500.011.618291
CGCGAAC11550.011.3939411
CTCAGAT164200.011.2490871
CGCAAAC45250.011.217681
CGCAAAT32800.011.176831
AAAAATC200000.010.8572
CTTTAAC77200.010.8367891
GTTTTCT213550.010.7623521
Produced by FastQC (version 0.11.5)