FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22723505
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG109500.056.2283194
CAAGTCG85100.026.3995394
TAAGTCG34450.024.420994
GAAGTCG130500.021.28505794
GTTTTTT302200.016.594641
TTTTTTC408650.016.0442922
AAAGTCG49150.015.87385694
CTCGATT36350.014.481431
CGGAAAT45900.013.6187371
TCGATTG20750.013.3638552
GTTTTTC175400.013.2371721
CGGATAT21050.013.1733981
TTTTTTG428000.012.9799072
CGAAAAT34500.012.9420281
CGCAAAT31400.012.722931
GTCGATT21900.012.4474891
GCCGAAT51000.012.4411761
GGAAAAT186150.012.2959981
GAAAAAT218250.012.0595661
GTTTTCT227000.011.9881061
Produced by FastQC (version 0.11.5)