FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22949733
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG105400.054.22390794
CAAGTCG90750.028.69201394
TAAGTCG29900.023.57859494
GAAGTCG121100.019.67712694
GTTCGAT28050.014.0748671
TTAGCGA23150.013.8056158
AATCGTA22250.013.7303375
AAAGTCG45950.013.60391794
TAGCGAG29650.012.5227669
ATCGTAT18950.011.6569936
GTCGGTT48900.011.4376281
TTCGATT31400.011.0764332
GTCGGAT26050.010.6449131
GGGAAAT166000.010.47590351
TACCGTA18550.010.388149
GTCGGAA38050.010.3758221
TATACGG14650.010.26621234-35
GTCCGAT15950.010.0188091
TAACGCA25000.09.96452-53
GGCGGAT93400.09.9635971
Produced by FastQC (version 0.11.5)