FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22949733
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG125750.045.63578494
CAAGTCG105200.027.61026694
TAAGTCG30550.024.61538594
GAAGTCG124100.017.04270794
AATCGTA23250.013.3419365
TTAGCGA19400.012.5979398
AAAGTCG46450.012.44564194
GTCGTTT23550.011.9745231
TAGCGAG25500.011.2431379
GTTCGAT26800.010.8731351
GCCGGTT55700.010.7163371
CGCGGTT40950.010.4444451
ATCGTAT17100.010.4444456
GGCGATT37100.010.134771
GGGAAAT155050.09.8819721
GGCGAAT36850.09.6933521
TTCGATT29750.09.6369742
CGCGTTT16600.09.6265061
GTCGGGT43200.09.4652781
GCGGTAT21550.09.378191
Produced by FastQC (version 0.11.5)