FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22792309
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG156750.039.87878894
CAAGTCG110200.023.24410294
TAAGTCG38950.023.16816394
GAAGTCG139050.019.80726494
CGCATAT6100.014.6393441
TACGCTA10050.014.4975139
GTTTTTT268400.014.0264531
TATACGC9400.014.07
CTTAGAT68950.013.8375641
CGCAAAC46350.013.4865141
CGCAATT22550.013.130821
CGAAAAC44450.013.0056241
CGAATCG16250.012.7261533
GTTTAAT82400.012.7196611
AAATCGA29450.012.6078094
CGAAAAT44050.012.5902391
CGAAGAT80100.012.5568041
AAAGTCG66650.012.27006794
GGAAAAT163800.012.1373621
CTTTAAC73350.012.1104291
Produced by FastQC (version 0.11.5)