FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21399860
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG86150.047.40917294
CAAGTCG72950.023.58053494
TAAGTCG29450.023.46010494
GAAGTCG106150.017.71078794
TCGGCTT63450.014.02
GTCGTTT23200.012.7629311
CGCGTAT3501.20549594E-412.0857141
GTCCGAT10550.011.5829381
CGCTTAA30350.011.14991844-45
CGTATCG11950.011.0125533
AAAGTCG43250.010.97572294
GTCGGTT46300.010.9632831
CTCGGTT36500.010.8164391
GGCGAAT58550.010.3552521
GTTTATT107050.010.317611
CGGCTTT73450.010.110283
GTCGAAA30850.010.0551061
CGCATTT33700.09.9020771
CTCAGAT164150.09.8495281
CGCGGAT20150.09.7965271
Produced by FastQC (version 0.11.5)