FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23061444
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG122950.055.96421494
CAAGTCG93350.029.35297294
TAAGTCG35150.025.1379894
GAAGTCG141600.021.74081894
AAAGTCG52000.015.54615494
GTTTTTT317750.015.1317071
TTTTTTC405200.014.2902272
GTTTAAT100200.012.7115761
AAATCGA32250.012.679074
GCGGTAT28650.012.4677141
TTTTTTG433300.012.4198022
GCGAAAT30700.012.4006521
CGCAAAT32600.012.3987731
GTTTTAC69400.012.3256481
TATACGC11850.012.295367
GCCGATT22800.011.751
ATATACG15000.011.5933346
CGAAAAT37050.011.54385951
GGAAAAT177700.011.4259991
TTTTTTA394750.011.3347692
Produced by FastQC (version 0.11.5)