FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21218096
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGC8850.016.4632787
GTTTTTT295550.015.743531
CGAAAAC34100.015.4369521
CGAAAAT41450.014.5138721
TACGCTA8250.014.2424269
CGCAATT18600.014.1505381
CGCAAAT30150.013.0945281
TCGAATC17300.012.7687862
GTTTAAT85250.012.6252211
GTTTTTC163550.012.443291
GTCGAAT12850.012.070041
CGCATAT7401.2732926E-1112.0675681
CTTTAAT122750.011.9462341
CGCGAAT7501.6370905E-1111.9066671
GTTTTAC73450.011.9019751
GTTTTAT155900.011.8178321
GCGAAAT22850.011.7242891
TTTAATC106700.011.5848182
CTTGAAC188800.011.5508481
GGAAAAT166800.011.46822551
Produced by FastQC (version 0.11.5)