FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22064254
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG111100.037.22772694
CAAGTCG84300.021.74377494
TAAGTCG29450.019.15110494
GAAGTCG106200.015.9764694
CGCGAAT6550.015.7862611
GTTTTTT303300.015.0778111
GCGAAAT25400.014.8031511
CTCGATT38900.014.1362481
CGCAAAT32500.014.0276931
CGAAAAC39050.013.2394361
CGCAAAC39850.012.855711
CGGAAAT41550.012.6690731
AAATCGA38600.012.6632154
CTTGAAT118250.012.2021141
CTTTAAT120400.012.1794021
CGCGTTT13150.012.1520911
GTTTTAT157900.012.114631
AAATCCA199300.011.9563474
CTCAGAT187250.011.8723641
CTCGGTT39200.011.8698981
Produced by FastQC (version 0.11.5)