FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23005502
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG108150.053.27970594
CAAGTCG93950.029.11548694
TAAGTCG34300.025.62390594
GAAGTCG114050.018.2560394
AATCGTA23050.012.0303695
AAAGTCG45550.011.96926594
GTTCGAT28150.011.6873881
GTCCGAT15600.011.4487181
GGCGAAT48850.010.8720571
GTCGGTT44600.010.854261
ATCGTAT18550.010.388146
GCCGATT23100.010.1731611
TTAGCGA19950.010.1303258
GGGAAAT160100.010.1280461
GTCGGAA36300.09.9696971
CGCGTTT13300.09.8947371
GTCGAAT15350.09.7980461
GCCGGTT52050.09.7521611
GTCCGTT26200.09.6870231
GTCGTTT21600.09.3564821
Produced by FastQC (version 0.11.5)