FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23005502
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG131850.043.7383494
CAAGTCG107350.027.45132894
TAAGTCG32850.019.88736794
GAAGTCG118950.018.76838994
GTCCGAT17650.013.8470261
CGACAAT20350.011.5479121
GTCGGTT41700.011.3836941
GTTCGAT26350.011.2371921
GTCGTTT24700.011.0364371
AAAGTCG52250.010.88421194
CGTATCG17550.010.7122513
GCCGGTT51400.010.33268451
GGGAAAT155200.010.2358261
GGAAAAT195500.09.7125321
CGCGTAA7302.0899643E-79.6575341
CTCGGTT37900.09.5488131
GGCGAAT44000.09.1863641
GCCGATT22600.09.1504421
GTCGGAT33950.08.9985281
GGCGGTT52900.08.8846881
Produced by FastQC (version 0.11.5)