FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22613372
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG103250.058.0842694
CAAGTCG90000.030.02777794
TAAGTCG31500.028.05079394
GAAGTCG117000.021.00940194
AAAGTCG45700.013.67833694
TTAGCGA19400.013.0824748
GTTCGAT23700.012.6919821
GCGGTAT20850.012.1726611
GTCCGAT15850.012.1577281
ATCGTAT19200.011.5052076
AATCGTA26000.011.3884615
TAGCGAG24800.011.1814519
GTCGGGT40750.010.7263791
GTCGCTT26900.010.6579921
GGGAAAT159750.010.5327061
GGCGAAT52250.010.4344491
GTCGGTT45000.010.0266661
GTCGGAA36900.09.9349591
CTCGGTT35750.09.860141
CGGAAAT47750.09.8429321
Produced by FastQC (version 0.11.5)