FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22613372
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG126750.047.2039494
CAAGTCG103550.027.46016194
TAAGTCG30950.025.81583294
GAAGTCG118200.019.12605794
AATCGTA20650.014.3389815
AAAGTCG53450.014.24508894
ATCGTAT17450.012.1203446
GGCGATT39250.012.0942681
CGCGGAT26450.011.9054811
CGCGTAA6051.3280442E-811.6528921
GTTCGAT25150.011.3996021
GTCGTTT23700.011.1054851
CGGGAAT52700.011.0588231
CGTATCG17250.010.898553
CGGGGTT90950.010.6454091
GGGAAAT154200.010.3326841
CGCGTAT5506.870323E-610.2545441
CGCGCAT15250.010.1704921
TTAGCGA21300.010.1502348
CGTAACG5153.7381134E-510.0388353
Produced by FastQC (version 0.11.5)