FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22207750
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG125550.045.59617694
CAAGTCG102550.025.61969894
TAAGTCG35200.024.0340994
GAAGTCG118650.018.85545594
AAAGTCG56300.015.44404894
GGCGAAT39700.011.8387911
CGCGATT7552.0008883E-1111.8278141
CGAGAAT35200.011.7499991
AATCGGC20500.011.0048775
CGCGTTT13300.010.9548861
CGCGAAT3903.294151E-410.8461531
CGAAGAT79250.010.793691
GGAAAAT207450.010.7389731
GTTTTAT155750.010.4410911
GTTTTTT292150.010.1834671
TCGGATT25600.010.0976552
GTCGTTT22350.010.0939591
TCGGATA19150.010.0626622
CGGAAAT40700.010.0466821
CTCGGAT55250.010.0380091
Produced by FastQC (version 0.11.5)