FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21972993
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG65850.038.47076494
CAAGTCG51050.020.89911794
TAAGTCG23400.017.67521394
GTTTATT110950.015.4195581
GTCGGTT55650.014.0197651
GTCGAAT13400.012.9776121
CGAATCG13450.012.9293673
GTTTTAT163900.012.5314221
GTTCGAT31550.012.513471
GTTTAAT104750.012.383771
AATCGTA19150.012.2715395
GTTTTCT227300.012.0549931
CGGAAAT45600.011.956141
CGCAAAC38400.011.751
CTTTATT157800.011.6755381
CGCGTAA6101.4999387E-811.5573761
GTCCGTT21650.011.5057731
GTCAATT66000.011.393941
CGAAAAT38900.011.35732651
GTTTTAC64400.011.239131
Produced by FastQC (version 0.11.5)