FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21972993
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG89700.028.29431294
TAAGTCG23750.018.0084294
CAAGTCG66850.017.29543794
CGCGTAT4806.6065695E-913.7083331
GTTTATT113400.013.4285711
CGCGTAA5503.2250682E-912.8181821
GTTTTAT153750.012.1665051
GTCGTTT21750.011.88505651
GTCGGTT49200.011.751
GTGAATT86150.011.40220451
TTAGCGA19450.011.35732657
GTAAAAC72150.011.2044341
AAATCGA29550.011.1336714
CGGAAAT38500.011.109091
CTTTAAT104500.010.9741621
GTTTTTT283650.010.96915151
GAAGTCG87150.010.9477994
GGAAAAT165600.010.8985511
CGCAAAC43250.010.8670521
GTTCGAT34250.010.8408751
Produced by FastQC (version 0.11.5)