FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22138010
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG105550.056.8185794
CAAGTCG87700.030.27936294
TAAGTCG30200.025.05629294
GAAGTCG119250.018.36645794
AATCGTA33600.016.7857135
GTCCGAT15850.015.7160881
ATCGTAT29300.015.2389096
ATAATCG31250.014.73923
AAAGTCG46800.014.56196594
TAATCGT31150.014.4847514
TCGTATC30000.013.9433337
CGTATCA29950.013.1819718
GTTCGAT24750.012.9131321
CGCTTAA37500.012.09466744-45
GTCGGGT43450.012.0069041
GGCGAAT46200.011.9025971
GCGGTAT22650.011.2052971
GTCGGTT42700.011.1170971
GCCGGTT47950.010.1939531
GTCGGAT31600.010.1139241
Produced by FastQC (version 0.11.5)