FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22138010
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG128900.046.89061494
CAAGTCG102850.027.69275794
TAAGTCG32550.020.93702194
AATCGTA30000.017.0766665
GAAGTCG115350.016.62418794
ATCGTAT24650.015.8255586
AAAGTCG53300.014.54971994
GTCCGAT15250.013.8688531
CGCTTAA40450.013.82694644-45
TCGTATC25400.013.6929137
TAATCGT27900.013.1397854
CGCGTAT4002.2553613E-612.9251
GGCGAAT41600.011.2980771
GGCGATT33450.011.2406581
CGCGGAT22350.011.1454141
ATAATCG33850.010.8301333
CACGCTT54600.010.71703242-43
CGCGTAA7051.1039447E-810.6666671
GTCGTTT25600.010.64843751
CTCGGTT34900.010.63896751
Produced by FastQC (version 0.11.5)